NDLI: A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris

Content Provider	Springer Nature Link
Author	Hang, Hai feng Chen, Wen Guo, Mei jin Chu, Ju Zhuang, Ying ping Zhang, Siliang
Copyright Year	2008
Abstract	Based on the fact that Pichia cell growth follows a Monod equation under the condition of methanol concentration limitation, a kinetics model of recombinant methylotrophic yeast Pichia pastoris expressing porcine insulin precursor (PIP) was developed in the quasi-steady state in the induction phase. The model revealed that the relationship between specific growth rate (µ) and substrate methanol concentration was in accord with the Monod equation. The fermentation kinetic parameters maximum specific growth rate (µ$_{ max }$), saturation constant (K$_{ s }$) and maintenance coefficient (M) were estimated to be 0.101 h$^{−1}$, 0.252 g l $^{−1}$, and 0.011 g MeOH g$^{−1}$ DCW h$^{−1}$, respectively. The unstructured model was validated in methanol induction phase with different initial cell densities. Results showed that the maximum specific protein production rate (q$_{ p.max }$) of 0.098 mg g$^{−1}$ DCW h$^{−1}$ was achieved when µ was kept at 0.016 h$^{−1}$, and the maximum yield of PIP reached 0.97 g l $^{−1}$, which was 1.5-fold as that of the control. Therefore, the simple Monod model proposed has proven to be a robust control system for recombinant porcine insulin precursor production by P. pastoris on pilot scale, which would be further applied on production scale.
Starting Page	1065
Ending Page	1069
Page Count	5
File Format	PDF
ISSN	02561115
Journal	Korean Journal of Chemical Engineering
Volume Number	25
Issue Number	5
e-ISSN	19757220
Language	English
Publisher	Springer US
Publisher Date	2009-01-09
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pichia pastoris Porcine Insulin Precursor Monod Modeling Methanol Feeding Strategy Biotechnology Materials Science Catalysis Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

High-expression of recombinant human concensus interferon-α by Pichia pastoris

Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shift

Improvement of ATP regeneration efficiency and operation stability in porcine interferon-α production by Pichia pastoris under lower induction temperature

Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris

Expression and purification of beefy meaty peptide in Pichia pastoris

Quantitative comparison of dynamic physiological feeding profiles for recombinant protein production with Pichia pastoris

Secretory expression of insulin precursor in pichia pastoris and simple procedure for producing recombinant human insulin.

Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance

Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris

A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris

Similar Documents

High-expression of recombinant human concensus interferon-α by Pichia pastoris

Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shift

Improvement of ATP regeneration efficiency and operation stability in porcine interferon-α production by Pichia pastoris under lower induction temperature

Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris

Expression and purification of beefy meaty peptide in Pichia pastoris

Quantitative comparison of dynamic physiological feeding profiles for recombinant protein production with Pichia pastoris

Secretory expression of insulin precursor in pichia pastoris and simple procedure for producing recombinant human insulin.

Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance

Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris

A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris