NDLI: Determination of meaty peptide in enzymatic hydrolyzate of beef protein by HPLC-MS

Content Provider	Springer Nature Link
Author	Zeng, Songrong Wang, Yanping Bai, Xiaojia Lin, Weili Yang, Ming Xing, Haipeng
Copyright Year	2008
Abstract	The purpose of this study is to detect beefy meaty peptide (BMP) in beef hydrolyzate. The synthesized BMP is used as a standard sample in the study. High performance liquid chromatography (HPLC)/ion trap electrospray ionization mass spectrometry (ESI-MS) with UV detection was applied in qualitative analysis of the peptides. Six beef protein enzymatic hydrolyzate samples were separated on a Surveyor HPLC system through a SUPELCO Discovery® C$_{18}$ analytical column (5 μm, 15 cm×2.1 mm i.d.). The column was eluted at a flow rate of 0.2 mL/min in a linear gradient elution mode of acetronitrile-water solution with 0.1% trifluoroacetic acid. The concentration of acetronitrile was increased from 5% to 50% in 40 minutes. A Finnigan LCQ Advantage MAX instrument was used as detector to analyze with ESI-MS and ESI-MS/MS in positive mode. Among the six samples of beef protein enzymatic hydrolysate, the BMP is detected and confirmed in sample No.4 with a higher intensity of characteristic peak and is further investigated by ESI-MS/MS. As a result, BMP exists in sample No.4. The study proves that HPLC-ESI-MS/MS is a simple, rapid, sensitive method to analyze target peptides from complex polypeptides.
Starting Page	1120
Ending Page	1124
Page Count	5
File Format	PDF
ISSN	02561115
Journal	Korean Journal of Chemical Engineering
Volume Number	25
Issue Number	5
e-ISSN	19757220
Language	English
Publisher	Springer US
Publisher Date	2009-01-09
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Beef Enzymatic Hydrolysis Beefy Meaty Peptide (BMP) HPLC-MS Biotechnology Materials Science Catalysis Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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