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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jung, You Ree Shin, Hyun Yong Yoo, Hah Young Um, Youngsoon Kim, Seung Wook |
| Copyright Year | 2012 |
| Abstract | To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and β-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T.reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel’s fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of β-glucosidase, compared with the wild type strain. Additionally, the properties of high level β-glucosidase produced by MT-2 were the same as those of the wild type strain, e.g., an optimum pH of 4.8, and an optimum temperature of 65 °C. Moreover, the protein concentrations of β-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then β-glucosidase activities were detected by the MUG-zymogram assay. |
| Starting Page | 925 |
| Ending Page | 930 |
| Page Count | 6 |
| File Format | |
| ISSN | 02561115 |
| Journal | Korean Journal of Chemical Engineering |
| Volume Number | 29 |
| Issue Number | 7 |
| e-ISSN | 19757220 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2012-02-07 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cellulases β-Glucosidase Trichoderma reesei Proton Beam Irradiation Mutation Industrial Chemistry/Chemical Engineering Catalysis Biotechnology Materials Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Chemical Engineering |
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