NDLI: The factors affecting the backward-transfer of Bovine Serum Albumin (BSA) from sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micellar solutions

Content Provider	Springer Nature Link
Author	Rho, Seon Gyun Kang, Choon Hyoung
Copyright Year	2003
Abstract	The factors affecting the back-extraction efficiency of Bovine Serum Albumin (BSA, 65kDa, pI 4.9) solubilized in an AOT reverse micellar solution, prepared by the injection method, to an excess aqueous phase were investigated. In particular, effects of pH, the type of salt and its concentration in the excess aqueous phase were examined. Furthermore, by comparing CD spectra of the back-extracted BSA with the feed BSA, the structural changes of the protein during the extraction process were determined. The addition of 1:1 salt such as KCl or NaCl to the aqueous phase resulted in a 100% recovery of the protein to the aqueous phase at a pH higher than its isoelectric point pI. This high efficiency of the back-extraction might be due to the change in the interactions between the protein and micellar aggregates driven by the added salt. For 1 : 2 salts like MgCl$_{2}$ or CaCl$_{2}$, BSA was back extracted with lower than 20% extraction efficiency. Maximum efficiencies were achieved at about pH=7 and pH=8 for monovalent and divalent salts, respectively. From the C D spectra of back-extracted BSA, it was observed that denaturation of BSA was not significant during the extraction process.
Starting Page	517
Ending Page	521
Page Count	5
File Format	PDF
ISSN	02561115
Journal	Korean Journal of Chemical Engineering
Volume Number	20
Issue Number	3
e-ISSN	19757220
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Reverse Micelle AOT Injection Method Back-Extraction BSA Industrial Chemistry/Chemical Engineering Catalysis Materials Science Biotechnology
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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