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| Content Provider | Springer Nature Link |
|---|---|
| Author | Liu, Chenguang Desai Kashappa, Goud H. Chen, Xiguang Park, Hyun Jin |
| Copyright Year | 2005 |
| Abstract | Chitosan was modified by conjugating coupling with linolenic acid through the l-ethyl-3-(3-dimethylaminopropyyl) carbodiimide (EDC)-mediated reaction. The degree of substitution 1.8% (i.e. 1.8 linolenic acid group per 100 anhydroglucose units) was measured by $^{1}$H NMR. The critical aggregation concentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value in phosphate-buffered saline (PBS) solution (pH7.4) was 5 × 10$^{−2}$ mgmL$^{−1}$. The average particle size of self- aggregates of hydrophobically modified chitosan in PBS solution (pH7.4) was 210.8 nm with a unimodal size distribution ranging from 100 to 500 nm. Transmission electron microscopy (TEM) study showed that the formation of near spherical shape nanoparticles has enough structural integrity. The loading ability of hydrophibically modified chitosan (LA-chitosan) was investigated by using bovine serum albumin (BSA) as the model. The loading capacity of self-aggregated nanoparticles increases (19.85% ±0.04% to 37.57% ±0.25%) with the concentration of BSA (O.l–O.5 mgmL$^{−1}$) |
| Starting Page | 234 |
| Ending Page | 239 |
| Page Count | 6 |
| File Format | |
| ISSN | 16725182 |
| Journal | Journal of Ocean University of China |
| Volume Number | 4 |
| Issue Number | 3 |
| e-ISSN | 19935021 |
| Language | English |
| Publisher | Science Press |
| Publisher Date | 2005-01-01 |
| Publisher Place | Beijing |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | chitosan linolenic acid nanoparticles self-aggregates BSA Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oceanography Ocean Engineering |
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