NDLI: Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

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Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

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Journal of Ocean University of China : Volume 7, Issue 1, February 2008

Journal of Ocean University of China : Volume 6

Journal of Ocean University of China : Volume 5

Journal of Ocean University of China : Volume 4

Journal of Ocean University of China : Volume 3

Journal of Ocean University of China : Volume 2

Journal of Ocean University of China : Volume 1

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

Content Provider	Springer Nature Link
Author	Wang, Jun Tang, Qingjuan Yue, Zhiqin Li, Zhaojie Zhang, Jin Xue, Changhu
Copyright Year	2008
Abstract	We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commercial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The results showed that NLVs in the four isolates belong to genogroup II. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient’s fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×10$^{8}$, 1.25×10$^{8}$ and 4.7×10$^{1}$ respectively. The detecting limit of NLVs was 1×10$^{1}$ copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.
Starting Page	223
Ending Page	227
Page Count	5
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	7
Issue Number	2
e-ISSN	19935021
Language	English
Publisher	SP Science Press
Publisher Date	2008-05-23
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	oysters Norwalk-like viruses (NLVs) reverse transcription polymerase chain reaction (RT-PCR) sequence analysis real time quantitative PCR Oceanography
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

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