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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhan, Aibin Bao, Zhenmin Wang, Mingling Chang, Dan Yuan, Jian Wang, Xiaolong Hu, Xiaoli Liang, Chengzhu Hu, Jingjie |
| Copyright Year | 2008 |
| Abstract | The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of H $_{ o }$ and H $_{ e }$ varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification. |
| Starting Page | 219 |
| Ending Page | 222 |
| Page Count | 4 |
| File Format | |
| ISSN | 16725182 |
| Journal | Journal of Ocean University of China |
| Volume Number | 7 |
| Issue Number | 2 |
| e-ISSN | 19935021 |
| Language | English |
| Publisher | SP Science Press |
| Publisher Date | 2008-05-23 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | microsatellite Pacific abalone Haliotis discus EST Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oceanography Ocean Engineering |
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