NDLI: Improved Methods for the Fatty Acid Analysis of Blood Lipid Classes

Content Provider	Springer Nature Link
Author	Ichihara, Ken’ichi Yoneda, Kumiko Takahashi, Ayuko Hoshi, riko Matsuda, Motoyoshi
Copyright Year	2011
Abstract	Two improved methods have been developed for preparation of fatty acid methyl esters (FAME) from major O-ester lipid classes in blood, i.e., cholesterol ester, triacylglycerol, and glycerophospholipids. The methods involve simple operations, and use neither harmful solvents such as chloroform or benzene nor highly reactive volatile reagents such as acetyl chloride. The FAME synthesis reaction proceeds under mild temperature conditions. The methods include (1) extraction of lipids from 0.2 ml of blood with 0.2 ml of tert-butyl methyl ether and 0.1 ml of methanol, (2) separation of the total lipids into lipid classes using a solid-phase extraction column or thin-layer chromatography, and (3) methanolysis of each lipid class at room temperature or at 45 °C. In all the operations, solvent concentration is performed only once prior to gas–liquid chromatography (GC). No noticeable differences in composition determined by GC have been found between FAME prepared by the present methods and those prepared by a conventional method involving lipid extraction with chloroform/methanol. The mild reaction and simplified procedures of the present methods enabled safe and reproducible analysis of the fatty acid compositions of the major ester-lipid classes in blood.
Starting Page	297
Ending Page	306
Page Count	10
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	46
Issue Number	3
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-07
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fatty acid composition Fatty acid methyl ester Methanolysis Cholesterol ester Plasma Phospholipid Triacylglycerol Blood lipids Medical Biochemistry Neurochemistry Medicinal Chemistry Lipidology Nutrition Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Preparation of Fatty Acid Methyl Esters by Selective Methanolysis of Polar Glycerolipids

High Saturated Fat Diet Alters the Lipid Composition of Triacylglycerol and Polar Lipids in the Femur of Dam and Offspring Rats

Fatty Acid Methyl Ester Profiles of Bat Wing Surface Lipids

Acetate Treatment Increases Fatty Acid Content in LPS-Stimulated BV2 Microglia

Metabolic Syndrome Affects Fatty Acid Composition of Plasma Lipids in Obese Prepubertal Children

Aberrations in Plasma Phospholipid Fatty Acids in Lung Cancer Patients

Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Prenatal Ethanol Exposure Increases Brain Cholesterol Content in Adult Rats

Lipid Droplets: Formation to Breakdown

Improved Methods for the Fatty Acid Analysis of Blood Lipid Classes

Similar Documents

Preparation of Fatty Acid Methyl Esters by Selective Methanolysis of Polar Glycerolipids

High Saturated Fat Diet Alters the Lipid Composition of Triacylglycerol and Polar Lipids in the Femur of Dam and Offspring Rats

Fatty Acid Methyl Ester Profiles of Bat Wing Surface Lipids

Acetate Treatment Increases Fatty Acid Content in LPS-Stimulated BV2 Microglia

Metabolic Syndrome Affects Fatty Acid Composition of Plasma Lipids in Obese Prepubertal Children

Aberrations in Plasma Phospholipid Fatty Acids in Lung Cancer Patients

Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Prenatal Ethanol Exposure Increases Brain Cholesterol Content in Adult Rats

Lipid Droplets: Formation to Breakdown

Improved Methods for the Fatty Acid Analysis of Blood Lipid Classes