NDLI: Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Content Provider	Springer Nature Link
Author	Metherel, Adam H. Aristizabal Henao, Juan J. Ciobanu, Flaviu Taha, Ameer Y. Stark, Ken D.
Copyright Year	2015
Abstract	Dried blood spots (DBS) by fingertip prick collection for fatty acid profiling are becoming increasingly popular due to ease of collection, minimal invasiveness and its amenability to high-throughput analyses. Herein, we assess a microwave-assisted direct transesterification method for the production of fatty acid methyl esters (FAME) from DBS. Technical replicates of human whole blood were collected and 25-μL aliquots were applied to chromatography strips prior to analysis by a standard 3-h transesterification method or microwave-assisted direct transesterification method under various power (variable vs constant), time (1–5 min) and reagent (1–10 % H$_{2}$SO$_{4}$ in methanol) conditions. In addition, a standard method was compared to a 5-min, 30-W power microwave in 1 % H$_{2}$SO$_{4}$ method for FAME yield from whole blood sphingomyelin, and sphingomyelin standards alone and spiked in whole blood. Microwave-assisted direct transesterification yielded no significant differences in both quantitative (nmol/100 µL) and qualitative (mol%) fatty acid assessments after as little as 1.5- and 1-min reaction times, respectively, using the variable power method and 5 % H$_{2}$SO$_{4}$ in methanol. However, 30-W power for 5 min increased total FAME yield of the technical replicates by 14 %. This increase appears largely due to higher sphingomyelin-derived FAME yield of up to 109 and 399 % compared to the standard method when determined from whole blood or pure standards, respectively. In conclusion, microwave-assisted direct transesterification of DBS achieved in as little as 1-min, and 5-min reaction times increase total fatty acids primarily by significantly improving sphingomyelin-derived fatty acid yield.
Starting Page	895
Ending Page	905
Page Count	11
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	50
Issue Number	9
e-ISSN	15589307
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-08-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fingertip prick Whole blood Microwave Direct transesterification Sphingomyelin Fatty acid methyl ester Sulfuric acid Lipidology Neurochemistry Medical Biochemistry Nutrition Medicinal Chemistry Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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