NDLI: Incorporation of Arachidonic and Stearic Acids Bound to L-FABP into Nuclear and Endonuclear Lipids from Rat Liver Cells

Content Provider	Springer Nature Link
Author	Maté, Sabina M. Layerenza, Juan P. Ves Losada, Ana
Copyright Year	2007
Abstract	The incorporation of exogenous fatty acids bound to L-FABP into nuclei was studied. Rat liver cell nuclei and nuclear matrices (membrane depleted nuclei) were incubated in vitro with [1-$^{14}$C]18:0 and 20:4n-6 either free or bound to L-FABP, ATP and CoA. FA esterification in whole nuclei and endonuclear lipids was ATP-CoA-dependent, and with specificity regarding fatty acid type and lipid class. 18:0 and 20:4n-6, free or L-FABP bound, showed the same incorporation and esterification pattern in lipids of whole nuclei. Only 20:4n-6 L-FABP bound was less incorporated into TAG with respect to free 20:4n-6. In the nuclear matrix, 18:0 free or L-FABP bound was esterified with a higher specific activity (SA) into: PtdEtn > PtdIns, PtdSer > PtdCho. 20:4n-6 free or L-FABP bound was esterified into: PtdIns > PtdEtn > PtdCho. 20:4n-6:L-FABP was esterified in endonuclear total-PL and PtdIns with a greater SA with respect to free 20:4n-6 and with a minor one as FFA. To summarize, trafficking of FA to nuclei includes esterification of 18:0 and 20:4n-6 either free or L-FABP-bound, into nuclear and endonuclear lipids by an ATP-CoA-dependent pathway. Endonuclear fatty acid esterification was more active than that in whole nuclei, and independent of the nuclear membrane. Esterification patterns of fatty acids L-FABP-bound or free into whole nuclear lipids were the same whereas in the nuclear matrix, L-FABP could play an important role in the mobilization of 20:4n-6 into specific sites of utilization such as the PtdIns pools.
Starting Page	589
Ending Page	602
Page Count	14
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	42
Issue Number	7
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-06-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell nuclei Nuclear matrix Endonuclear lipids Arachidonic acid Stearic acid Fatty acid incorporation L-FABP Medical Biochemistry Bioorganic Chemistry Nutrition Microbial Genetics and Genomics Medicinal Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The incorporation of fatty acids of different chain length into liver and biliary lipids in the perfused rat liver

Incorporation and distribution of saturated and unsaturated fatty acids into nuclear lipids of hepatic cells

Incorporation of n−3 fatty acids into plasma and liver lipids of rats: Importance of background dietary fat

Role of Fatty Acid Binding Proteins and Long Chain Fatty Acids in Modulating Nuclear Receptors and Gene Transcription

Important Differences Exist in the Dose–Response Relationship between Diet and Immune Cell Fatty Acids in Humans and Rodents

Heneicosapentaenoate (21:5n−3): Its incorporation into lipids and its effects on arachidonic acid and eicosanoid synthesis

A $^{13}$C nuclear magnetic resonance study of free fatty acid incorporation in acylated lipids in differentiating predipocytes

Comparative effects of α- and γ-linolenic acids on rat liver fatty acid oxidation

Incorporation of polyunsaturated fatty acids into CT-26, a transplantable murine colonic adenocarcinoma

Incorporation of Arachidonic and Stearic Acids Bound to L-FABP into Nuclear and Endonuclear Lipids from Rat Liver Cells

Similar Documents

The incorporation of fatty acids of different chain length into liver and biliary lipids in the perfused rat liver

Incorporation and distribution of saturated and unsaturated fatty acids into nuclear lipids of hepatic cells

Incorporation of n−3 fatty acids into plasma and liver lipids of rats: Importance of background dietary fat

Role of Fatty Acid Binding Proteins and Long Chain Fatty Acids in Modulating Nuclear Receptors and Gene Transcription

Important Differences Exist in the Dose–Response Relationship between Diet and Immune Cell Fatty Acids in Humans and Rodents

Heneicosapentaenoate (21:5n−3): Its incorporation into lipids and its effects on arachidonic acid and eicosanoid synthesis

A $^{13}$C nuclear magnetic resonance study of free fatty acid incorporation in acylated lipids in differentiating predipocytes

Comparative effects of α- and γ-linolenic acids on rat liver fatty acid oxidation

Incorporation of polyunsaturated fatty acids into CT-26, a transplantable murine colonic adenocarcinoma

Incorporation of Arachidonic and Stearic Acids Bound to L-FABP into Nuclear and Endonuclear Lipids from Rat Liver Cells