NDLI: Exogenous Sphingosine 1-Phosphate Protects Murine Splenocytes Against Hypoxia-Induced Injury

Content Provider	Springer Nature Link
Author	Chawla, Sonam Sahni, Chayanika Tulsawani, Rajkumar Singh, Mrinalini Saraswat, Deepika Bansal, Anju Saxena, Shweta
Copyright Year	2013
Abstract	Sphingosine-1-phosphate (S1P), a biologically active pleiotropic lipid, is involved in several physiological processes especially in the area of vascular biology and immunology encompassing cell survival, angiogenesis, vascular tone, immune response etc. by interacting with specific cell surface receptors. Hypoxia, a condition common to innumerable pathologies, is known to lethally affect cell survival by throwing off balance global gene expression, redox homeostasis, bioenergetics etc. Several molecular events of cellular adaptations to hypoxia have been closely linked to stabilization of hypoxia inducible factor-1α (HIF-1α). Signalling functions of S1P in physiological events central to hypoxia-induced pathologies led us to investigate efficacy of exogenous S1P in preconditioning murine splenocytes to sustain during cellular stress associated with sub-optimal oxygen. The present study recapitulated the pro-survival benefits of exogenous S1P under normobaric hypoxia. Results indicate a direct effect of S1P supplementation on boosting cellular adaptive responses via HIF-1α stabilization and, activation of pro-survival mediators ERK and Akt. Overwhelming anti-oxidative and anti-inflammatory benefits of S1P preconditioning could also be captured in the present study, as indicated by improved redox homeostasis, reduced oxidative damage, balanced anti/pro-inflammatory cytokine profiles and temporal regulation of nitric oxide secretion and intra-cellular calcium release. Hypoxia induced cell death and the associated stress in cellular milieu in terms of oxidative damage and inflammation could be alleviated with exogenous S1P preconditioning.
Starting Page	191
Ending Page	202
Page Count	12
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	49
Issue Number	2
e-ISSN	15589307
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-11-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sphingosine-1-phosphate Pro-survival Hypoxia Anti-oxidant Anti-inflammatory HIF-1α Lipidology Neurochemistry Medical Biochemistry Nutrition Medicinal Chemistry Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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