NDLI: TLC and $^{31}$P-NMR Analysis of Low Polarity Phospholipids

Content Provider	Springer Nature Link
Author	Vyssotski, Mikhail MacKenzie, Andrew Scott, Dawn
Copyright Year	2008
Abstract	High-performance TLC and $^{31}$P-NMR were assessed as methods of observing the presence of numerous low polarity phospholipids: bis-phosphatidic acid (BPA), semi-lyso bis-phosphatidic acid (SLBPA), N-acyl phosphatidylethanolamine (NAPE), N-(1,1-dimethyl-3-oxo-butyl)-phosphatidylethanolamine (diacetone adduct of PE, DOBPE), N-acetyl PE, phosphatidylmethanol (PM), phosphatidylethanol (PEt), phosphatidyl-n-propanol (PP), phosphatidyl-n-butanol (PB). Both techniques are non-discriminative and do not require the prior isolation of individual lipids. It appears that 2D TLC is superior to $^{31}$P NMR in the analysis of low polarity phospholipids. All phosphatidylalcohols were well separated by 2D TLC. However, some compounds which can present difficulty in separation by 2D-TLC (e.g., SLBPA and NAPE; or DOBPE and N-acetyl PE) were easily distinguished using $^{31}$P NMR so the methods are complimentary. A disadvantage of 2D TLC is that Rf values can vary with different brands and batches of TLC plates. The chemical shifts of $^{31}$P NMR were less variable, and so a library of standards may not be necessary for peak identification. Another advantage of $^{31}$P NMR is the ease of quantification of phospholipids. The applicability of the methods was tested on natural extracts of fish brain and cabbage stem.
Starting Page	381
Ending Page	389
Page Count	9
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	44
Issue Number	4
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-12-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	$^{31}$P-NMR TLC Phosphatidylmethanol Phosphatidylethanol N-acetone adduct Phosphatidylpropanol Phosphatidylbutanol Bis-phosphatidic acid Acyl phosphatidylglycerol N-acyl phosphatidylethanolamine Microbial Genetics and Genomics Medicinal Chemistry Nutrition Medical Biochemistry Neurochemistry Lipidology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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