NDLI: Lipoprint Adequately Estimates LDL Size Distribution, but not Absolute Size, Versus Polyacrylamide Gradient Gel Electrophoresis

Content Provider	Springer Nature Link
Author	Varady, Krista A. Lamarche, Beît
Copyright Year	2011
Abstract	Recently, a new cost-effective and less labor-intensive technique termed the “lipoprint LDL system” was developed to measure LDL particle size. However, the agreement between lipoprint and previously validated techniques, such as polyacrylamide gradient gel electrophoresis (PGGE), has never been tested. Therefore, we measured LDL size by lipoprint and PGGE in 16 obese subjects at 4 different time points. Lipoprint significantly overestimated (P = 0.003) integrated LDL particle size by 1.1 ± 3.0 Å when compared to PGGE. As for distribution, there was good agreement between methods for the estimation of large, medium, and small particles (mean difference between the methods was <3% for each parameter). Correlational analysis also revealed good relationships between methods for the proportion of large (r = 0.81, P < 0.0001), medium (r = 0.67, P < 0.0001), and small (r = 0.73, P < 0.0001) particles. In sum, although there is good agreement between lipoprint and PGGE for the determination of LDL size distribution, absolute LDL size values may differ between the two methods.
Starting Page	1163
Ending Page	1167
Page Count	5
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	46
Issue Number	12
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	LDL particle size Validation Lipoprint Quantimetrix Polyacrylamide gradient gel electrophoresis Medical Biochemistry Microbial Genetics and Genomics Lipidology Nutrition Neurochemistry Medicinal Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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