NDLI: A Novel Approach for Determination of Free Fatty Acids in Vegetable Oils by a Flow Injection System with Manual Injection

Content Provider	Springer Nature Link
Author	Ayyildiz, H. Filiz Kara, Huseyin Sherazi, S. T. H.
Copyright Year	2011
Abstract	A non-aqueous flow injection method for determining free fatty acid (FFA) content in corn and sunflower oil samples was developed. A single-line manifold system was built by modification of an HPLC for flow injection analysis (FIA). Without pre-treatment, oil samples were injected into a n-propanol solution containing KOH and phenolphthalein (PHP). The main parameters, such as flow rate of carrier phase, length, geometry, inner diameters of the coils and reagent concentration were all optimized. The proposed FIA method was validated for precision, accuracy, linear region, limit of detection (LOD) and limit of quantification (LOQ). The intra- and inter-day measurements of the precision of the method were found to be within the limits of acceptance criteria (RSD < 1%), and were rugged when the method was performed by a different analyst. The linear concentration range was calculated as 0.09–1.50 and 0.07–1.40 FFA% for corn and sunflower oils, correspondingly. The LOD and LOQ were found to be 7.53 × 10$^{−4}$–2.28 × 10$^{−3}$ oleic acid % and 7.11 × 10$^{−4}$–2.23 × 10$^{−3}$ oleic acid % for corn and sunflower oils, respectively. The results were compared with those obtained by the AOCS (Ca-5a-40) method using statistical t and F tests, and a significant difference was not observed between the methods at a 95% confidence level. The proposed method is suitable for quality control of routine applications due to its simplicity, high sample throughput, and economy of solvents and sample, offering considerable promise as a low cost analytical system that needs minimum human intervention over long periods of time.
Starting Page	1181
Ending Page	1190
Page Count	10
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	46
Issue Number	12
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flow injection analysis (FIA) Vegetable oils Free fatty acid (FFA) Direct analysis Corn oil Sunflower oil Medical Biochemistry Microbial Genetics and Genomics Lipidology Nutrition Neurochemistry Medicinal Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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