NDLI: Propagation of Gentiana macrophylla (Pall) from hairy root explants via indirect somatic embryogenesis and gentiopicroside content in obtained plants

Content Provider	Springer Nature Link
Author	Wu, Hai Jun Wang, Xin Xin Li, Yan Zhang, Dong Guang Zhang, Bing Wang, Xin Yu
Copyright Year	2011
Abstract	An effective protocol for plant regeneration from hairy root (HR) via indirect somatic embryogenesis was established in medicinal plant Gentiana macrophylla, a perennial herb in Gentianaceae. On the MS medium containing 0.5–2.5 mg l$^{−1}$ 2,4-dichlorophenoxyacetic acid (2,4-D) or 2,4-D plus benzylaminopurine (BAP), all the HR explants produced embryogenic calli (ECs). After transfer to plant growth regulator (PGR)-free MS medium, up to 94% of the ECs produced somatic embryos (SEs) of various stages, including cotyledonary SEs. When the calli with cotyledonary SEs were transferred to PGR-free MS medium, the cotyledonary SEs on the calli developed into plantlets (1–12 ones per callus). The cotyledonary SEs showed two types: solitary and fasciculate. The former developed into single plantlets and the latter into fasciculate ones. After transplantation into soil, a half of the plantlets survived, and one of the survivors flowered without fruiting. Morphologically, about 30% plantlets appeared similar to the wild type (WT)-plants, and 70% of them displayed wrinkled dark green leaves with relatively small and dense stomata, long and thick main root with dense lateral roots. The biomass of roots and leaves of the plantlets increased by five- and one-fold, respectively, and the content of gentiopicroside of their roots raised by 72.4%, in comparison with WT-plants. Polymerase chain reaction revealed that the rolC gene integrated into HR genome still existed in the regenerated plants. This study offers us an effective method and material for producing gentiopicroside or other medicinal compounds.
Starting Page	2229
Ending Page	2237
Page Count	9
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	33
Issue Number	6
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-04-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomass Medicinal plants Regeneration in vitro metabolism Somatic embryo Agriculture Plant Pathology Plant Physiology Plant Anatomy/Development Plant Biochemistry Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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