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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mikuła, Anna Skierski, Janusz Rybczyński, Jan J. |
| Copyright Year | 2002 |
| Abstract | Experiments were carried out with three-year-old embryogenic suspension culture of Gentiana pannonica Scop. The initial explant for the suspension determinated both the embryogenic character and embryo production. Cultures were initiated by culture of hypocotyl, cotyledon and root explants on MS (Murashige and Skoog 1962) medium supplemented with 1.0 mg·l$^{−1}$ Kinetin and 0.5 mg·l$^{−1}$ 2,4-D, later transferred and maintained in liquid MS medium with 1.0 mg·l$^{−1}$ Dicamba, 0.1 mg·l$^{−1}$ NAA, 2.0 mg·l$^{−1}$ BAP and 80.0 mg·l$^{−1}$ AS. Regeneration medium included 0.0–1.0 mg·l$^{−1}$ GA$_{3}$+0.0−2.0 mg·l$^{−1}$ Kin.+0.0−160 mg·l$^{−1}$ AS.In these culture conditions, the effect of the explant was found to be the most important factor. The curve of growth, growth coefficient and % of participation of various size aggregates differed in the studied suspensions. Flow cytometry revealed various DNA content in nuclei from praembryogenic mass depending on the explant origin. To complete embryogenesis the medium was changed from liquid to solidified in the presence of the same plant growth regulators combination required. The most embryogenic culture appeared hypocotyl-derived and it yielded the highest number of somatic embryos. The suspension culture originating from root proliferated the highest number of embryogenic cell clusters but did not produce embryos for fraction 120–450 µm. One hundred mg of suspension of the fraction that was larger than 450 µm yielded 309, 175, 123 embryos for the following suspensions: root-, cotyledon-, hypocotyl-derived, respectively. Almost 50 % of non-deformed fully developed embryos from all studied suspensions passed conversion into germling stage and finally plants were regenerated. |
| Starting Page | 311 |
| Ending Page | 322 |
| Page Count | 12 |
| File Format | |
| ISSN | 01375881 |
| Journal | Acta Physiologiae Plantarum |
| Volume Number | 24 |
| Issue Number | 3 |
| e-ISSN | 18611664 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2002-01-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Gentiana pannonica seedling explants cell suspension long term culture somatic embryogenesis germlings flow cytometry Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Plant Science Agronomy and Crop Science |
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