NDLI: Identification of differentially expressed genes preferably related to drought response in pigeon pea (Cajanus cajan) inoculated by arbuscular mycorrhizae fungi (AMF)

Content Provider	Springer Nature Link
Author	Qiao, Guang Wen, Xiaopeng Yu, Lifei Ji, Xiangbiao
Copyright Year	2012
Abstract	Pigeon pea is an ideal crop for sustainable agriculture systems in Karst areas of southwest China, which frequently suffers from the formidable water deficit. Physiologically, arbuscular mycorrhizae (AM)-colonized pigeon pea (Cajanus cajan) demonstrated a further enhanced tolerance to drought stress. To elucidate the molecular mechanism underlying the elevated tolerance, suppression subtractive hybridization (SSH) were employed to dig up the differentially expressed genes using mixed cDNAs prepared from drought-stressed and unstressed pigeon pea seedlings inoculated by AM fungi (AMF) in the present work. Both forward and reverse SSH cDNA library were constructed and a total of 768 clones were obtained. Dot-blotting expression analysis identified that 142 clones were upregulated, and 49 were downregulated during water stress. After sequencing, 182 unique expressed sequence tags (ESTs) were obtained via blast analysis, among which 142 (78%) exhibited high homology to previously identified or putative proteins, however, 40 (22%) showed no homology in the database. The upregulated (102) and downregulated (40) ESTs with significant protein homology might be sorted into 16 and 12 functional categories respectively, which involved in a broad spectrum of biological pathways. Furthermore, semi-quantitative reverse transcription (RT)-PCR was carried out for the 35 differentially expressed genes whose putative functions implicated in abiotic stress tolerances in other species, and it was verified these differentially expressed genes highly involved in drought stress tolerance of AM-colonized pigeon pea.
Starting Page	1711
Ending Page	1721
Page Count	11
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	34
Issue Number	5
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-02-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arbuscular mycorrhizae Differentially expressed genes Drought stress Pigeon pea (Cajanus cajan) Suppression subtractive hybridization (SSH) Agriculture Plant Pathology Plant Biochemistry Plant Anatomy/Development Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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