NDLI: Differential expression of expressed sequence tags in alfalfa roots under aluminum stress

Content Provider	Springer Nature Link
Author	Fan, Fu Li, Xin Wei Wu, Yue Ming Xia, Zhuo Sheng Li, Jin Jun Zhu, Wen Liu, Jin Xin
Copyright Year	2010
Abstract	To gain a better understanding of differentially expressed sequence tags (ESTs) for aluminum (Al) tolerance and to investigate the molecular mechanisms of Al toxicity, cDNA subtraction libraries were generated from Al-stressed roots of alfalfa (Medicago sativa L.) compared with no Al-stressed ones, employing suppression subtractive hybridization. Using differential screening technique in which the probes were labeled with DIG, we identified 45 non-redundant ESTs in Al-stressed alfalfa root tips with significantly altered expression. Among the up-regulated ESTs, we have found genes encoding identified proteins, including malate dehydrogenase, 6-phosphogluconate dehydrogenase, peroxidase, and an ABC transporter, while the down-regulate genes included ATPase, secretory carrier membrane protein 2, pectinesterase inhibitor. In addition, two novel ESTs, EW678752 and EY976957, up- and down-regulated by Al stress were sequenced. Analyzed by real-time PCR, the expressions of EST EW678718, EW678739, EY976969 and EW678728, which encode for ABC transporter, malate dehydrogenase, peroxidase and 6-phosphogluconate dehydrogenase correspondingly, increased 1.64-, 2.75-, 3.27- and 6.54-folds, respectively, and the expression of EY976957 encoding for ATPase decreased 3.27 folds. The expression of EST EW678752 increased 34.54-fold, while that of EY976957 decreased 16.68 folds. It suggested that the two novel ESTs maybe play a significant role in the aluminum tolerance of alfalfa.
Starting Page	539
Ending Page	546
Page Count	8
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	33
Issue Number	2
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Al toxicity Alfalfa (Medicago sativa L.) Expressed sequence tag (EST) Suppression subtractive hybridization (SSH) Agriculture Plant Physiology Plant Biochemistry Plant Genetics & Genomics Plant Pathology Plant Anatomy/Development
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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