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Increasing NaCl and CaCl$_{2}$ concentrations in the growth medium of quince leaves: I. Effects on somatic embryo and root regeneration

Increasing NaCl and CaCl$_{2}$ concentrations in the growth medium of quince leaves: II. Effects on shoot regeneration

In Vitro Cellular & Developmental Biology - Plant : Volume 38, Issue 3, May 2002

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Increasing NaCl and CaCl$_{2}$ concentrations in the growth medium of quince leaves: II. Effects on shoot regeneration

Content Provider	Springer Nature Link
Author	D'ofrio, C. Morini, S.
Copyright Year	2002
Abstract	The effects of NaCl and CaCl$_{2}$ on shoot regeneration from quince (Cydonia oblonga BA L29 clone) leaves were investigated. Caulogenesis was induced on in vitro-grown leaves treated for 2d in liquid Murashige and Skoog (MS) medium with 11.3 μM 2,4-dichlorophenoxyacetic acid and cultured on MS gelled medium supplemented with 4.5 μM thidiazuron and 0.5 μM naphthaleneacetic acid. Three experiments were performed: in the first, we compared the effects of NaCl at 0, 25, 50, 100, and 200 mM in factorial combination with 3, 9, and 27 mM CaCl$_{2}$. In the second, NaCl was tested at 0, 5, 10, 20, 40, and 80 mM with CaCl$_{2}$ at 0.3, 1.0, and 3.0 mM. The third experiment was carried out with the same experimental design as the second one but replacing NaCl with Na$_{2}$SO$_{4}$. Shoot regeneration was evaluated after 50 d of culturing: 25 in darkness and 25 in white light. In the first experiment, shoot regeneration was very poor and was observed only at the lower salt concentrations. In the second experiment, the percentages of caulogenic leaves were much higher, but decreased with increasing NaCl concentration. The more pronounced negative effect of the highest NaCl concentrations appeared to be partly mitigated by CaCl$_{2}$ at 1 and 3 mM. The presence of 3 mM CaCl$_{2}$, in the experiment with Na$_{2}$SO$_{4}$, appeared to be even more effective in reducing the adverse effect of sodium stress on caulogenesis. This result was attributed to the lower Cl$^{−}$ concentration in the growth medium, which resulted from replacing NaCl with Na$_{2}$SO$_{4}$. NaCl applied at low concentrations (5 and 10 mM) in combination with 3 mM CaCl$_{2}$ exerted a favorable effect on adventitious shoot regeneration. As regards the Na$^{+}$ and Ca$^{2+}$ interaction, when the Na$^{+}$/Ca$^{2+}$ ratio was below roughly 35 and 20, with NaCl and Na$_{2}$SO$_{4}$, respectively, at least 60% of leaves showed regenerating capacity, but optimal values of this ratio were not derived.
Starting Page	373
Ending Page	377
Page Count	5
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	38
Issue Number	4
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	caulogenesis Cydonia oblonga in vitro culture saline stress Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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