NDLI: Micropropagation and hairy root culture of Solanum Laciniatum Ait.

Content Provider	Springer Nature Link
Author	Okršlar, Veronika Štrukelj, Borut Kreft, Samo Bohanec, Borut Zel, Jana
Copyright Year	2002
Abstract	An efficient system for in vitro micropropagation of Solanum laciniatum Ait. has been established. Shoot induction on leaf explants was most successful on Murashige and Skoog (MS) medium supplemented with 10 μM N$^{6}$-benzyladenine (BA) and 1 μM α-naphthaleneacetic acid (NAA). BA (13 μM) was optimal for further shoot multiplication, and rooting of separated shoots was achieved on medium without plant growth regulators. At each subculture, 20–25 shoots were obtained on each explant, from which six to eight were suitable for separation and further rooting. Leaf explants grown in vitro were successfully infected by Agrobacterium rhizogenes ATCC 15834. The established hairy root culture was, on the basis of dry weight, more productive when grown on half-strength MS medium than on full-strength MS (3% sucrose) and full-strength MS (6% sucrose) medium. The amount of solasodine-containing glycoalkaloids in hairy roots as measured by a colorimetric method was 0.3–1% of dry weight, which is higher than in the shoot culture (0.5% of dry weight) and lower than in leaves of in vivo-grown plants (1.1–1.4% of dry weight). The amount of solasodine-containing glycoalkaloids in leaves of in vivo-grown plants of S. laciniatum was similar to the related species Solanum aviculare Forst. Both species are morphologically similar, therefore we effectively distinguished them by flow cytometry. The genome size of S. laciniatum was determined as 4.03 pg and the genome size of S. aviculare as 1.69 pg.
Starting Page	352
Ending Page	357
Page Count	6
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	38
Issue Number	4
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Solanum laciniatum Ait solasonine solasodine shoot culture transformed root culture steroidal alkaloids Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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