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| Content Provider | Springer Nature Link |
|---|---|
| Author | Rana, Satiander Dhar, Niha Bhat, Wajid W. Razdan, Sumeer Khan, Shabnam Dhar, Rekha S. Dutt, Prabhu Lattoo, Surrinder K. |
| Copyright Year | 2012 |
| Abstract | Withania somnifera, commonly known as ashwagandha or Indian ginseng, is a valuable medicinal plant, synthesizing a wide array of pharmacologically active secondary metabolites known as withanolides. In this study, we investigated variation among 54 regenerated plants attained through indirect organogenesis from leaf explants. Organogenic calli were induced on Murashige and Skoog medium containing 2 mg l$^{−1}$ kinetin and 1 mg l$^{−1}$ indole-3-butyric acid. High-performance liquid chromatography was used for quantitative determination of the major withanolides in the somaclones. One somaclone (WS-R-1) showed significantly higher accumulation of 12-deoxywithastramonolide (WS-12D; 0.516%) compared to the explant donor mother plant (0.002%). The incidence of somaclonal variation at the cytological level was investigated by studying mitosis and meiosis in relation to chromosome number and structural organization. There were no alterations in chromosome phenotypes, somatic chromosome count, or meiotic behavior. Fidelity at genomic level was evaluated by random amplification of polymorphic DNA (RAPD) analyses, which revealed multiple genetic polymorphisms between the WS-12D over-producing somaclone and the explant donor mother plant. This study demonstrates the capability of inducing chemotypic variability for the development of high-yielding clones due to molecular instability in W. somnifera using an in vitro approach. |
| Starting Page | 546 |
| Ending Page | 554 |
| Page Count | 9 |
| File Format | |
| ISSN | 10545476 |
| Journal | In Vitro Cellular & Developmental Biology - Plant |
| Volume Number | 48 |
| Issue Number | 5 |
| e-ISSN | 14752689 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-09-01 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Withania somnifera Somaclone Withanolides 12-Deoxywithastramonolide Cytogenetic analysis Chemovariant Plant Genetics & Genomics Plant Breeding/Biotechnology Cell Biology Plant Sciences Developmental Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Plant Science Biotechnology |
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