NDLI: In vitro propagation of the Damask rose (Rosa damascena Mill.)

Content Provider	Springer Nature Link
Author	odezh, Haleh Mahmoudi Moieni, Ahmad Baghizadeh, Amin
Copyright Year	2012
Abstract	Roses are an important commercial crop available in a wide range of varieties in international markets. Due to its economic value, this study aimed to establish a new and reproducible protocol for the in vitro propagation of Rosa damascena Mill. We developed an efficient and cost-effective method for rapid and high-quality shoot multiplication and in vitro rooting of Damask rose using nodal explants. For each stage of the micropropagation procedure (i.e., explant establishment, shoot multiplication and growth, and rooting), different media and combinations of plant growth regulators were utilized. A new culture medium, termed A$_{19}$, resulted in significant improvements to shoot proliferation and root induction for this rose cultivar. For optimal explant establishment, shoot growth, and proliferation, a modified Murashige and Skoog medium with higher levels of nitrates, calcium, and iron plus supplementation with 4 mg/l 6-benzylaminopurine and 0.25 mg/l indole-3-acetic acid was utilized. To increase shoot length, 75 d after culture initiation (including two subcultures), shoots were transferred to the same medium additionally supplemented with 0.2 mg/l gibberellic acid. This resulted in vigorous shoot growth, with longer shoots and a greater number of shoots per explant. Shoots were then separated and transferred to various root induction medium for 30 d. The results clearly showed that a liquid ½A$_{19}$ medium-A (i.e., with half-strength macroelements) supplemented with 0.1 mg/l indole-3-butyric-acid was the most successful medium for in vitro rooting in this cultivar. Shoots were cultured in this medium for 7 d in the dark, before transfer to liquid ½A$_{19}$ medium-A without hormone supplementation under a 16-h photoperiod. This modified protocol resulted in significant improvement in shoot regeneration and proliferation and obtained stronger shoots over a period of about 20 wk.
Starting Page	530
Ending Page	538
Page Count	9
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	48
Issue Number	5
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rosa damascena Mill. Damask rose Micropropagation In vitro rooting Plant Genetics & Genomics Plant Breeding/Biotechnology Cell Biology Plant Sciences Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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