NDLI: Foliar nutrition of in vitro-cultured Prosopis chilensis (Molina) Stuntz shoots

Content Provider	Springer Nature Link
Author	Mandair, N. S. Harris, P. J. C.
Copyright Year	2003
Abstract	Foliar nutrition has been conceived as a possible means of overcoming the recalcitrance of Prosopis chilensis (Molina) Stuntz explants to standard in vitro culture. The foliar uptake of cations (K from 20 gl$^{−1}$ KNO$_{3}$ and Ca from 50 gl$^{−1}$ CaCl$_{2}$), anions (NO$_{3}$ from 50 gl$^{−1}$ KNO$_{3}$ and PO$_{4}$ from 50 gl$^{−1}$ NaH$_{2}$PO$_{4}$), and glucose from a 100 mg l$^{−1}$ solution studied. All of the nutrients examined were absorbed. The efficacy of foliar nutrition in prolonging the vigor of micropropagated P. chilensis shoot tips was compared with nutrients supplied as a liquid to the base of the stem (liquid) or as an agar-solidified medium (agar). A foliar-feeding apparatus was constructed that employed pressurization of the medium reservoir to drive the medium into the culture vessel with a passive return by a siphoning effect. The medium used was Murashige and Skoog with 30 gl$^{−1}$ sucrose, 0.1 mgl$^{−1}$ benzylaminopurine, and 1 mgl$^{−1}$ indole-3-butyric acid. Over a 9-wk test period it was found that explants cultured by foliar nutrition performed significantly better than those grown on agar for shoot length, nodal production, and leaf retention; and better than liquid MS for node production. There was no significant difference among the three treatments in percentage survival, percentage rooting, or the mean number of roots.
Starting Page	240
Ending Page	244
Page Count	5
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	39
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	foliar nutrition shoot culture prosopis chilensis Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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