NDLI: Photoautotrophic growth of sugarcane plantlets in vitro as affected by photosynthetic photon flux and vessel air exchanges

Content Provider	Springer Nature Link
Author	Xiao, Yulan Lok, Yee Hin Kozai, Toyoki
Copyright Year	2003
Abstract	Explants of sugarcane, a C$_{4}$ plant, were cultured in vitro for 18d on Floridalite (a solid cube consisting of vermiculite and cellulose fibers) used as supporting material with sugar-free Murashige and Skoog liquid medium with double-strength KH$_{2}$PO$_{4}$, MgSO$_{4}$, FeSO$_{4}$, and Na$_{2}$-EDTA in the vessel with enhanced natural ventilation. CO$_{2}$ concentration in the culture room was kept at 1500 μmol mol$^{−1}$ (four times the atmospheric CO$_{2}$ concentration) during the photoperiod. A factorial experiment was designed with two levels of photosynthetic photon flux (PPF) and three levels of N (number of air exchanges of the vessel). The results were compared with those in the control treatment (photomixotrophic culture using sugar-containing agar medium under low PPF and low N). PPF and N showed significant positive effects on the growth of sugarcane plantlets in vitro. In the photoautotrophic (using sugar-free medium) treatments with relatively high PPF (200–400 μmol m$^{−2}$ s$^{−1}$) and high N (2–10 h$^{−1}$), the growth of plantlets was four to seven times greater than that in the control. Also, the culture period for multiplication and rooting was shortened from 30 d in the control to 18 d or less in the photoautotrophic, high PPF, and high N treatments. Use of porous supporting material in photoautotrophic treatments promoted rooting and plantlet growth significantly.
Starting Page	186
Ending Page	192
Page Count	7
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	39
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	C$_{4}$ plant CO$_{2}$ enrichment microporous supporting material ventilation Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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