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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Zhifen Finer, John J. |
| Copyright Year | 2014 |
| Abstract | Soybean (Glycine max) promoters from an actin gene (GmActin), a ribosomal protein S11 gene (GmRpS11) and a heat shock protein 90 gene (GmHsp90), identified as being active during early induction of soybean somatic embryogenesis, were cloned, fused to the green fluorescence protein (gfp) gene and reintroduced into soybean for evaluation. All promoters displayed development- and tissue-specific regulation based on the expression of GFP in transgenic soybean. In seedlings, these three promoters gave rise to strong GFP expression in young roots, with very high levels of expression in the vascular tissues and root primordia. In older plants, promoter activity was observed in the vascular cambium of petioles, leaf midribs, and in the mesophyll of expanding leaves, but at reduced levels. The GmActin promoter gave very high GFP expression in developing and mature seeds. All three promoters displayed high activity following induction of somatic embryogenesis from immature soybean cotyledons. Comparison of promoter-regulated GFP transgene expression with transcriptome analysis using RNA-Seq revealed agreement in expression intensity in roots, leaves, and flowers but exceptions for expression in young developing seeds. Evaluation of specific promoters in transgenic plants is needed for proper elucidation of promoter-regulated transgene activity. |
| Starting Page | 9 |
| Ending Page | 18 |
| Page Count | 10 |
| File Format | |
| ISSN | 10545476 |
| Journal | In Vitro Cellular & Developmental Biology - Plant |
| Volume Number | 51 |
| Issue Number | 1 |
| e-ISSN | 14752689 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2014-12-06 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Promoter Tissue-specificity Transgene expression Soybean development gfp Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Plant Science Biotechnology |
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