Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Kumar, Suresh Sahu, Neha Singh, Archana |
| Copyright Year | 2015 |
| Abstract | Cenchrus ciliaris L. is an important perennial forage grass that grows throughout the semi-arid tropics. It reproduces predominantly by apomixis, which provides a means of clonal propagation through seeds. The absence of sexual reproduction in C. ciliaris limits the possibilities of genetic improvement by hybridization. A rare obligate sexual plant of C. ciliaris (IGFRI-CcSx-08/1) that is self-incompatible in nature requires vegetative propagation to maintain its genotype. In vitro culture is one of the possible ways for clonal multiplication and a prerequisite for genetic manipulation. Here, we report high frequency in vitro plant regeneration via callus induction from immature inflorescences of this obligate sexual plant. Embryogenic calli were induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (3.5 mg L$^{−1}$), and shoot organogenesis was obtained on MS medium supplemented with kinetin (2.0 mg L$^{−1}$). Rooting from the regenerated shoots was achieved on MS medium containing indolebutyric acid (2.0 mg L$^{−1}$) and activated charcoal (2.0 g L$^{−1}$). The survival rate of the plants under ex vitro conditions was 70%. Molecular analysis of the tissue cultured plants using sequence characterized amplified region (SCAR) markers and by embryo sac analysis revealed obligate sexual reproduction in all the regenerated plants. The tissue cultured plants were true to the mother plant type. The high frequency of in vitro plant regeneration achieved by this protocol would be very useful for clonal multiplication and genetic manipulation of this rare genotype of C. ciliaris. |
| Starting Page | 28 |
| Ending Page | 34 |
| Page Count | 7 |
| File Format | |
| ISSN | 10545476 |
| Journal | In Vitro Cellular & Developmental Biology - Plant |
| Volume Number | 51 |
| Issue Number | 1 |
| e-ISSN | 14752689 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2015-01-29 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Apomixis Forage grass Immature inflorescence SCAR marker Sexual reproduction Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Plant Science Biotechnology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|