NDLI: Shoot regeneration and ploidy variation in tissue culture of honeydew melon (Cucumis melo L. inodorus)

Content Provider	Springer Nature Link
Author	Ren, Yan Bang, Haejeen Gould, Jean Rathore, Keerti S. Patil, Bhimanagouda S. Crosby, Kevin M.
Copyright Year	2012
Abstract	The Cucumis melo L. inodorus honeydew melon variant is one of the most consumed melons in the USA, and has important commercial and nutritional value. There is a need for improvement of several genetic traits in the US honeydew melon, such as nutrition, drought tolerance, and disease resistance. We investigated the existing regeneration media and optimized the medium composition for an elite honeydew diploid breeding line, “150”, using cotyledonary explants. Four combinations of three different plant growth regulators, 6-benzyladenine, abscisic acid, and indole-3-acetic acid (IAA), were tested in the shoot regeneration media. The presence of IAA in the medium caused the cotyledon explants to curl away from the medium, which made antibiotic selection problematic in our previous study. Omission of IAA from the culture media eliminated this problem and did not impact the shoot regeneration capacity of the cotyledonary explants. We also estimated the ploidy of regenerated plants using flow cytometry, and 50–60% were found to be polyploid (tetraploid or mixoploid). However, contrary to other studies, these polyploid plants did not show major morphological differences compared to the diploid plants.
Starting Page	223
Ending Page	229
Page Count	7
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	49
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-12-21
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Honeydew melon Indole-3-acetic acid In vitro regeneration Polyploidy Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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