NDLI: A novel pathway for rapid shoot regeneration from the proximal zone of the inpocotyl of melon (Cucumis melo L.)

Content Provider	Springer Nature Link
Author	Curuk, S. Elman, C. Schlarman, E. Sagee, O. Shomer, I. Cetiner, S. Gray, D. J. Gaba, V.
Copyright Year	2002
Abstract	Hypocotyl explants of melon (Cucumis melo L.) are capable of regenerating multiple shoots on Murashige and Skoog (1962) medium, augmented with 4.4 μM benzylademne. Regeneration from the hypocotyl is much more rapid than the more commonly reported regeneration from cotyledonary explants, producing shoots within 2 wk compared to more than a month required for cotyledon explants. The rapid regeneration response depends on the presence of a fragment of the cotyledon remaining attached to the hypocotyl. Controls were performed to ensure that the regeneration was not due to the presence of the shoot apical bud of the melon seedling after explant production. Scanning electron microscopy revealed that microsurgery to remove the apical bud left no excess bud material. Regeneration from the proximal part of the hypocotyl was due to production of a new shoot apical meristem, observed by histology. The apical meristem can be produced before leaf primordia in regeneration from the hypocotyl, in contrast to the regeneration process from the melon cotyledon.
Starting Page	260
Ending Page	267
Page Count	8
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	38
Issue Number	3
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	organogenesis bud regeneration shoot apex cotyledon cucurbits Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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