NDLI: Ischemia hypothermia improved contractility under normothermia reperfusion in the model of cultured cardiomyocyte

Content Provider	Springer Nature Link
Author	Li, Heng Fang, Xiangshao Yang, Zhengfei Fu, Yue Wang, Yu Wu, Jinlang Yu, Tao Huang, Zitong
Copyright Year	2012
Abstract	Though mild hypothermia displays an optimistic alleviation of contractive failure in the ischemia/reperfusion myocardium, we still lacked answers to many questions about its potential mechanisms. Our hypothesis is that hypothermia (32°C) induced in ischemia can ease mitochondrial injury resulting in improvement of myocardial contractility even under the condition of a normothermic reperfusion. Fifty newly born 1–2 d Sprague–Dawley rats were executed and the primary cardiomyocytes were obtained and cultivated in vitro. Myocytes were randomized into three groups and then subjected to ischemia either at 32°C or 37°C, both prior to undergoing reperfusion at 37°C. Contractility was presented as frequency and velocity. Ultrastructural alterations of cardiomyocytes and mitochondrion underwent semi-quantitative analysis with transmission electron microscopy and respiratory function of mitochondria was further assessed simultaneously. During cooling ischemia and following reperfusion, cardiomyocytes acquired a more immediate restoration to baseline level and had a significant difference as compared with those in normothermia (P < 0.05). Furthermore, hypothermia preserved the ultrastructure of myocytes and mitochondrion after ischemia. However, measurement on Heart Injury Score and form factor revealed no differences after 2-h reperfusion either in hypothermia or normothermia. On the contrary, the surface area and respiratory function of mitochondrion in reperfusion differed significantly in both groups (P < 0.05) which had an accordance with the variation on contractile performance. Hypothermia only induced in ischemia can bring contractility benefit even under a normothermia reperfusion in cultured cardiomyocytes
Starting Page	284
Ending Page	292
Page Count	9
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	48
Issue Number	5
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-04-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Therapeutic hypothermia Cardiomyocytes Ischemia/reperfusion Contractility Ultrastructure Respiratory function Cell Culture Stem Cells Developmental Biology Cell Biology Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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Ischemia hypothermia improved contractility under normothermia reperfusion in the model of cultured cardiomyocyte