NDLI: NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic, or preosteoclastic pathway in vitro

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NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic, or preosteoclastic pathway in vitro

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NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic, or preosteoclastic pathway in vitro

Content Provider	Springer Nature Link
Author	Oguro, Akira Kawase, Tomoyuki Orikasa, Michiaki
Copyright Year	2003
Abstract	The stimulatory effects of sodium fluoride (NaF) on bone formation have been explained solely, by its activation of osteoblasts. However, whether and how NaF acts on the osteoclast linearge is poorly understood. We previously found that NaF differentiates HL-60 cells to granulocytic cells. To further test this action, we have employed here primary cultures of progenitor cells derived from murine bone marrow. NaF at subtoxic concentations (<0.5 mM) significantly up-regulated activities of several intracellular enzymes (lactate dehydrogenase, β-glucuronidase, acid phosphatase), cellular reduction of nitroblue tetrazolium, and nitric oxide (NO) production; which are all accepted as general differentiation markers. NaF (<0.5 mM) also up-regulated granulocyte-specific markers (chloroacetate esterase, cell surface antigens[Mac-1, Gr-1]) but not any of the monocyte-specific markers (nonspecific esterase, cell surface antigens [F4/80, MOMA-2]). Although other general differentiation markers (phagocytosis, adhesion, appearance, nuclear:cytoplasmic ratio) were not appreciably influenced by NaF, essentially in support of our prevous data from HL-60 cells, the present findings suggest that NaF induces early differentiation of bone marrow hemopoietic progenitor cells along the granulocytic pathway but not the monoicytic pathway that is linked to osteoclast formation. Therefore, in addition to its potent stimulatory effects on osteoblastic bone formation, NaF applied to patients with osteoporosis could be expected to indirectly reduce osteoclastic bone resorption.
Starting Page	243
Ending Page	248
Page Count	6
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	39
Issue Number	5-6
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	fluoride bone marrow cell granulocyte differentiation osteoclast monocyte Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic, or preosteoclastic pathway in vitro

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