NDLI: Ovarian primary tissue culture of the kuruma shrimp Marsupenaeus japonicus

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In Vitro Cellular & Developmental Biology - Animal : Volume 39, Issue 3-4, March 2003

In Vitro Cellular & Developmental Biology - Animal : Volume 39, Issue 1-2, January 2003

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In Vitro Cellular & Developmental Biology - Animal : Volume 37

In Vitro Cellular & Developmental Biology - Animal : Volume 36

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In Vitro Cellular & Developmental Biology - Animal : Volume 34

In Vitro Cellular & Developmental Biology - Animal : Volume 33

Ovarian primary tissue culture of the kuruma shrimp Marsupenaeus japonicus

Content Provider	Springer Nature Link
Author	Maeda, Miru Mizuki, Eiichi Itami, Toshiaki Ohba, Michio
Copyright Year	2003
Abstract	Cell growth in ovarian primary culture of the kuruma shrimp, Marsupenaeus japonicus, was examined under various culture conditions. The best growth of ovarian cells was obtained in a culture system consisting of double strength of Leibowitz-15 medium supplemented with 10% fetal bovine serum, glucose (1 g/L), proline (0.1 g/L), TC-Yeastolate (1 g/L), and lactalbumin hydrolysate (1 g/L). The cells survived in this medium at 25° C for 45 d. The epithelial-like cells predominated in 10-d-old cultures, covering >80% of the surface area on the bottom of flask. Cells in mitosis were often observed. Cell proliferation was monitored by incorporation of 5-bromo-2′-deoxyuridine (BrdU), an analog of thymidine. 5-Bromo-2′-deoxyuridine-associated cells accounted for 11.5 and 35.0% of cell populations at 2 and 24 h, respectively, after BrdU treatment. Our results provide an improved culture technique for ovarian tissue of the kuruma shrimp.
Starting Page	208
Ending Page	212
Page Count	5
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	39
Issue Number	5-6
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	prawn crustacean ovary cell culture cell growth BrdU Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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