NDLI: Establishment of rat brain endothelial cells susceptible to rat cytomegalovirus ALL-03 infection

Content Provider	Springer Nature Link
Author	Camalxaman, Siti Nazrina Zeenathul, Nazariah Allaudin Quah, Yi Wan Loh, Hwei San Zuridah, Hassan Hani, Homayoun Sheikh Omar, Abdul Rahman Mohd Azmi, Mohd Lila
Copyright Year	2013
Abstract	Endothelial cells have been implicated as key cells in promoting the pathogenesis and spread of cytomegalovirus (CMV) infection. This study describes the isolation and culture of rat brain endothelial cells (RBEC) and further evaluates the infectious potential of a Malaysian rat CMV (RCMV ALL-03) in these cultured cells. Brain tissues were mechanically fragmented, exposed to enzymatic digestion, purified by gradient density centrifugation, and cultured in vitro. Morphological characteristics and expression of von Willebrand factor (factor VIII-related antigen) verified the cells were of endothelial origin. RBEC were found to be permissive to the virus by cytopathic effects with detectable plaques formed within 7 d of infection. This was confirmed by electron microscopy examination which proved the existence of the viral particles in the infected cells. The susceptibility of the virus to these target cells under the experimental conditions described in this report provides a platform for developing a cell-culture-based experimental model for studies of RCMV pathogenesis and allows stimulation of further studies on host cell responses imposed by congenital viral infections.
Starting Page	238
Ending Page	244
Page Count	7
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	49
Issue Number	3
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-02-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytopathology Isolation Endothelial cells Rat cytomegalovirus Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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