NDLI: Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet

Content Provider	Springer Nature Link
Author	Wang, Sheng ping Gao, Yun ling Liu, Gang Deng, Dun Chen, Rong jun Zhang, Yu zhe Li, Li li Wen, Qing qi Hou, Yong qing Feng, Ze meng Guo, Zhao hui
Copyright Year	2015
Abstract	The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.通过pEnho 基因的克隆, 分析pEnho 基因在仔猪相关组织的分布情况, 增加仔猪糖脂代谢方面相关的知识, 为通过调控adropin 来促进仔猪的健康和生长提供理论基础。首次克隆了猪Enho 基因, 命名为pEnho, 其基因序列提交至美国国立生物技术信息中心 (NCBI) (No. GQ414763)。通过实时荧光定量逆转录聚合酶链式反应 (real-time RT-PCR) 方法获得猪Enho 基因序列, 并应用BLAST、Clustal W、PHYLIP、TMHMM、Helixturnhelix等生物信息学软件对其基因序列进行分析; 通过荧光定量PCR 方法检测了不同日龄 (出生后1、7、14 和21 天) 猪Enho 基因的组织分布 (肝脏、肌肉、空肠前端、空肠后端和回肠)。克隆得到pEnho 基因序列; 发现随着仔猪日龄的增加, 其表达量有下降; 在组织分布上, 产后7 天内pEnho 在肠道 (特别是回肠) 的表达量较高, 在第14 天和第21 天, 各被检组织的表达量无显著差异。
Starting Page	524
Ending Page	532
Page Count	9
File Format	PDF
ISSN	16731581
Journal	Journal of Zhejiang University SCIENCE B
Volume Number	16
Issue Number	6
e-ISSN	18621783
Language	Chinese
Publisher	Zhejiang University Press
Publisher Date	2015-05-13
Publisher Place	Hangzhou
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adropin Energy homeostasis-associated (Enho) gene Gene coloning Piglets RT-PCR Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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