NDLI: Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

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Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Content Provider	Springer Nature Link
Author	Lei, Kai Li, Ya li Wang, Yang Wen, Jing Wu, Hong zhao Yu, Dong you Li, Wei fen
Copyright Year	2015
Abstract	While a high-fat diet (HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1% (w/w) Bacillus subtilis B10 (HFD+B10 group). Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride (TG), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activities. In addition, the gene expressions of antioxidant genes, glutathione reductase (GR), xanthine oxidase (XO), heat-shock protein 90 (Hsp90), and lipid synthesis gene 3β-hydroxysteroid-Δ24 reductase (DHCR24) in the HFD+B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), energy metabolism gene peroxisome proliferator-activated receptor α (PPARα) and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice.探究枯草芽孢杆菌B10 对高脂日粮诱导的小鼠脂肪代谢及氧化应激的改善作用, 并初步探讨其作用机制。证明了枯草芽孢杆菌B10 可以有效改善高脂日粮诱导的小鼠脂肪代谢和氧化应激, 且发现此作用主要与B10 调节脂肪代谢基因 (PPARα 、DHCR24、HMGCS2) 及氧化应激基因 (XO、p53) 表达和谷胱甘肽过氧化物酶 (GSH-Px) 活力有关。将ICR 雄鼠分为对照组 (饲喂高脂日粮) 和实验组 (饲喂添加枯草芽孢杆菌菌粉的高脂日粮)。饲喂30 天后, 收集小鼠的血清及肝脏样品。采用试剂盒测定抗氧化及脂肪代谢相关指标和肝脏中8-羟基脱氧鸟苷 (8-OHdG) 含量。使用荧光定量聚合酶链式反应 (PCR) 测定小鼠肝脏中脂肪代谢和氧化应激相关基因的表达水平。饲喂含有枯草芽孢杆菌B10 的高脂日粮能够有效降低小鼠的体重 (表2), 降低血清中葡萄糖和甘油三酯含量及谷草转氨酶和谷丙转氨酶活力 (表3 和 4); 下调肝脏中脂肪合成相关基因表达量, 但上调脂肪分解相关基因表达量 (图1), 并提高肝脏中抗氧化相关基因表达量 (图2)。综上所述, 枯草芽孢杆菌B10 能有效调节小鼠脂肪代谢, 并改善其氧化应激。
Starting Page	487
Ending Page	495
Page Count	9
File Format	PDF
ISSN	16731581
Journal	Journal of Zhejiang University SCIENCE B
Volume Number	16
Issue Number	6
e-ISSN	18621783
Language	Chinese
Publisher	Zhejiang University Press
Publisher Date	2015-05-25
Publisher Place	Hangzhou
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacillus subtilis High-fat diet Oxidative stress Lipid metabolism Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice* #

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