NDLI: Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation

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Journal of Zhejiang University SCIENCE B : Volume 8, Issue 3, March 2007

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Journal of Zhejiang University SCIENCE B : Volume 8, Issue 1, January 2007

Journal of Zhejiang University SCIENCE B : Volume 7

Journal of Zhejiang University SCIENCE B : Volume 6

Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation

Content Provider	Springer Nature Link
Author	Wang, Guan yu Zhang, Jia wei Lü, Qing hua Xu, Rong zhen Dong, Qing hua
Copyright Year	2007
Abstract	To investigate the effect of berbamine on human hepatoma cell line SMMC7721.The effects of 24 h and 48 h incubation with different concentrations (0∼64 µg/ml) of the berbamine on SMMC7721 cells were evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Hoechst 33258 staining was conducted to distinguish the apoptotic cell, and the appearance of sub-G$_{1}$ stage was determined by PI (propidium iodide) staining, the percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Flow cytometry was performed to analyze the cell cycle distribution and the mitochondrial membrane potential (Δψ $_{m}$); the expression of activated caspase3 and caspase9 was analyzed by Western-blot.The proliferation of SMMC7721 was decreased after treatment with berbamine in a dose-and time-dependent manner. Berbamine could induce apoptosis in SMMC7721 cells and could cause cell cycle arrest in G$_{0}$/G$_{1}$ phase, to induce loss of mitochondrial membrane potential (Δψ $_{m}$) and activate caspase3 and caspase9. Berbamine-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk.Berbamine exerts antiproliferative effects on human hepatocellular carcinoma SMMC7721 cells. The anticancer activity of berbamine could be attributed partly to its inhibition of cell proliferation and induction of apoptosis in cancer cells through loss in mitochondrial transmembrane potential and caspase activation.
Starting Page	248
Ending Page	255
Page Count	8
File Format	PDF
ISSN	16731581
Journal	Journal of Zhejiang University SCIENCE B
Volume Number	8
Issue Number	4
e-ISSN	18621783
Language	English
Publisher	Zhejiang University Press
Publisher Date	2007-01-01
Publisher Place	Hangzhou
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Berbamine Apoptosis Mitochondrial membrane potential Caspase Hepatoma Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation*

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Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation

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