NDLI: The tasks and puzzles of cloning

Content Provider	Springer Nature Link
Author	Nikitin, V. A.
Copyright Year	2010
Abstract	The attention of researchers is drawn to the fact that, in spite of great effort put into mammalian cloning, advances in this area are quite limited, and cloned animals have developmental pathologies often incompatible with life and/or reproduction. It is not clear yet whether these shortcomings are underlain by biological or technical factors, or more likely both. There is a great number of papers dealing with the influence of cloning by nuclear transfer on genetic and epigenetic reprogramming of donor cells. At the same time, we see practically no analytical studies on the cloning procedure itself, its weak points, and possible sources of cell trauma during microsurgery of nuclear transfer or twinning. This article discusses, step by step, some nuclear transfer techniques and methods of splitting early preimplantation embryos for twinning, with the aim to reveal possible causes of cell injury in micromanipulation that would adversely affect the development of cloned organisms. Several new author’s technologies based on the study of cell biophysical characteristics are described, which allow avoiding or at least minimizing cell trauma.
Starting Page	386
Ending Page	393
Page Count	8
File Format	PDF
ISSN	00063509
Journal	Biophysics
Volume Number	55
Issue Number	3
e-ISSN	15556654
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-10-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cloning nuclear transfer microsurgery cell damage microinjection twinning Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Biophysics

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