NDLI: A comparative study of proliferation and osteogenic differentiation of rat adipose-derived stem cells in β-tricalcium phosphate (β-TCP), forsterite (Mg$_{2}$SiO$_{4}$) and clinoenstatite (MgSiO$

Content Provider	Springer Nature Link
Author	Leng, Bin Jin, XiaoGang Lin, QiuXia Chen, Lei Wang, Yan Du, ZhiYan Lin, KaiLi Chang, Jiang Gu, XiaoMing Wang, ChangYong
Copyright Year	2013
Abstract	In this study, the effects of forsterite and clinoenstatite powder extracts on the proliferation and osteogenic differentiation of rat adipose-derived stem cells (ASCs) were investigated and compared with the β-tricalcium phosphate (β-TCP) powder extracts. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and live-dead staining were performed to evaluate the viability and proliferation of rat ASCs. Osteogenic differentiation of rat ASCs were assayed by alkaline phosphatase (ALP) staining and ALP activity test. The expression of osteogenic marker genes (alkaline phosphatase (ALP), runt related transcription factor 2 (Runx2), collagen type Iα1 (Col1α1), secreted phosphoprotein1 (Spp1, osteopontin), integrin binding sialoprotein (Ibsp), bone gla protein (Bglap)) were detected by real-time polymerase chain reaction (PCR). The MTT assay and the live-dead staining showed that all the three ceramics possessed good cytocompatibility with rat ASCs. Furthermore, forsterite and clinoenstatite promoted the proliferation of rat ASCs compared with β-TCP. The results of the ALP activity test and the real-time PCR demonstrated that forsterite and clinoenstatite promoted the osteogenic differentiation of rat ASCs. These results suggested that forsterite and clinoenstatite are bioactive ceramics that may be used for preparation of bone tissue engineering (BTE) scaffolds.
Starting Page	3033
Ending Page	3042
Page Count	10
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	58
Issue Number	24
e-ISSN	18619541
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-05-11
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	forsterite clinoenstatite adipose-derived stem cells osteogenesis bone bone tissue engineering Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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