NDLI: Expression of reconstructive hFVIII in the hrDNA by using hrDNA targeting vector

Content Provider	Springer Nature Link
Author	Liu, Xionghao Liu, Mujun Hua, She Lu, Wen Xue, Zhigang Liang, Desheng Cai, Fang Pan, Qian Long, Zhigao Wu, Lingqian Dai, Heping Xia, Kun Xia, Jiahui
Copyright Year	2005
Abstract	Our lab has constructed a new nonviral vector—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression cassette of reconstructive hFVIII (hFVIII-BDDAK39) to pHrneo to construct targeting vector: pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFVIII-BDDAK39 in the hrDNA locus. The hFVIII-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific integration was 2.0×10$^{−5}$. hFVIII-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently (32±5 ng·10$^{6}$ cells$^{−1}$ · 24 h$^{−1}$). Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia.
Starting Page	2187
Ending Page	2192
Page Count	6
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	50
Issue Number	19
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2005-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	human derived vector site-specific integration targeting expression reconstructive hFVIII Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Non-viral ex vivo transduction of human hepatocyte cells to express factor VIII using a human ribosomal DNA-targeting vector

Expression of human clotting factor IX mediated by recombinant lentiviral vector in cultured cells and hemophilia B mice

Progress of gene targeting in mouse

Targeting strategies for adeno-associated viral vector

Mutational effect of the “- 35” element of sorghumpsbA gene promoter

Gene expression profiles of the developing human retina

Expression of inhibin a and βA subunits in cultured human first trimester trophoblast tissue

Investigating the function of Akt by tet-off inducible expression system

Expression of reconstructive hFVIII in the hrDNA by using hrDNA targeting vector

Similar Documents

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Non-viral ex vivo transduction of human hepatocyte cells to express factor VIII using a human ribosomal DNA-targeting vector

Expression of human clotting factor IX mediated by recombinant lentiviral vector in cultured cells and hemophilia B mice

Progress of gene targeting in mouse

Targeting strategies for adeno-associated viral vector

Mutational effect of the “- 35” element of sorghumpsbA gene promoter

Gene expression profiles of the developing human retina

Expression of inhibin a and βA subunits in cultured human first trimester trophoblast tissue

Investigating the function of Akt by tet-off inducible expression system

Expression of reconstructive hFVIII in the hrDNA by using hrDNA targeting vector