NDLI: Cloning and expression of AtPLC6, a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana

Content Provider	Springer Nature Link
Author	Xu, Xiaojing Cao, Zhixiang Liu, Guoqin Bhattacharrya, Madan K. Ren, Dongtao
Copyright Year	2004
Abstract	A full-length cDNA clone corresponding to a putative phosphatidylinositol-specific phospholipase C (PI-PLC) was isolated from Arabidopsis thaliana by screening a cDNA library and using RT-PCR strategy. The cDNA, designated AtPLC6, encodes a putative polypeptide of 578 amino acid residues with a calculated molecular mass of 66251.84 D and a pI of 7.24. The sequence analysis indicates that the polypeptide contains X, Y, EF-hand and C2 domains. The overall structure of putative AtPLC6 protein, like other plant PI-PLCs, is most similar to that of mammalian PLCS. The recombinant AtPLC6 protein expressed in E. coli was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (TP3) and 1,2-diacylglycerol (DAG). The protein hydrolyzes PIP2 in a Ca$^{2+}$-dependent manner and the optimum concentration of Ca$^{2+}$ is 10 μmol/L. These results suggested that AtPLC6 gene encodes a genuine PI-PLC. Northern blot analysis showed that the AtPLC6 gene is expressed at low level in all examined tissues, such as roots, stems, leaves, flowers, siliques and seedlings under normal growth conditions. The gene is strongly induced under low temperature and weakly induced under various stresses, such as ABA, high-salt stress and heat. These results suggested that AtPLC6 might be involved in the signal-trans-duction pathways of cold responses of the plants.
Starting Page	567
Ending Page	573
Page Count	7
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	49
Issue Number	6
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2004-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Arabidopsis thaliana AtPLC6 gene cloning expression in prokaryotic cell cold stress Life Sciences Physics Chemistry/Food Science Science Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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