NDLI: Genome sequencing and characterization analysis of a Beijing isolate of chicken coronavirus infectious bronchitis virus

Content Provider	Springer Nature Link
Author	Jin, Weiwu Chen, Chen Zhang, Ying Zhao, Yiqiang Feng, Jidong Chen, Fuyong Wu, Qingming Yang, Hanchun Wang, Ming Yu, Jialin Li, Ning Gong, Yuanshi Sun, Qixin Chen, Zhangliang
Copyright Year	2004
Abstract	Avian infectious bronchitis virus (AIBV) is classified as a member of the genus coronavirus in the family coronaviridae. The enveloped virus has a positive-sense, single-stranded RNA genome of approximately 28 kilo-bases, which has a 5′ cap structure and 3′ polyadenylation tract. The complete genome sequence of infectious bronchitis virus (IBV), Beijing isolate, was determined by cloning sequencing and primer walking. The whole genome is 27733 nucleotides in length, has ten open reading frames: 5′ -orf1a-orf1ab-s-3a-3b-e-m-6a-6b-n-3′. Alignments of the genome sequence of IBV Beijing isolate with those of two AIBV strains and one SARS coronavirus were performed respectively. The genome sequence of IBV Beijing isolate compared with that of the IBV strain LX4 (uncompleted, 19440 bp in size) was 91.2% similarity. However, the full-length genome sequence of IBV Beijing isolate was 85.2% identity to that of IBV Strain Beaudette, and was only 50.8% homology to that of SARS coronavirus. The results showed that the genome of IBV has remarkable variation. And IBV Beijing isolate is not closely related to SARS coronavirus. Phylogenetic analyses based on the whole genome sequence, S protein, M protein and N protein, also showed that AIBV Beijing isolate is lone virus in group III and is distant from SARS coronavirus. In conclusion, this study will contribute to the studies of diagnosis and diseases control on IBV in China.
Starting Page	585
Ending Page	590
Page Count	6
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	49
Issue Number	6
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2004-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	avian infectious bronchitis virus (AIBV) severe acute respiratory syndrome coronavirus (SARS-CoV) primer walking, sequence analysis Life Sciences Physics Chemistry/Food Science Science Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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