NDLI: Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton

Content Provider	Springer Nature Link
Author	Zhu, HuaGuo Tu, LiLi Jin, ShuangXia Xu, Li Tan, JiaFu Deng, FengLin Zhang, XianLong
Copyright Year	2008
Abstract	The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin.
Starting Page	3666
Ending Page	3676
Page Count	11
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	53
Issue Number	23
e-ISSN	18619541
Language	English
Publisher	SP Science in China Press
Publisher Date	2008-11-28
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	cellular dedifferentiation cotton proline-rich protein SAM-dependent transmethylation suppression subtractive hybridization Engineering Geosciences Science Chemistry/Food Science Physics Life Sciences
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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