NDLI: Identification of genes associated with cotyledon senescence in upland cotton

Content Provider	Springer Nature Link
Author	Shen, Fafu Yu, Shuxun Xie, Qingen Han, Xiulan Fan, Shuli
Copyright Year	2006
Abstract	In order to unravel the biochemical pathways and understand the molecular mechanisms involved in leaf senescence, suppression subtractive hybridization (SSH) was used to generate a cDNA library enriched for transcripts differentially expressed in developmental senescence cotyledons of upland cotton. After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis, selected 678 clones were sequenced and analyzed. Sequencing of these cDNA fragments reveals that 216 of expressed sequence tags (ESTs) represented unique genes. Of these 216 cDNAs, 151 clones (69.9%) show significant homologies to previously known genes, while the remaining 65 do not match any known sequences. 151 unique ESTs are assigned to twelve different categories based on their putative functions generated by BLAST analysis. These SAG-encoded proteins are likely to participate in macromolecule degradation, nutrient recycling, detoxification of oxidative metabolites, and signaling and regulatory events. The expression pattern of selection of genes was confirmed using northern hybridization. Northern hybridization confirmed several distinct patterns, from expression at a very early stage to the terminal phase of the senescence syndrome. Clones encoding proteases and proteins involved in macromolecule degradation and gluconeogenesis, as well as stress-related genes, are up regulated in senescence cotyledons.
Starting Page	1085
Ending Page	1094
Page Count	10
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	51
Issue Number	9
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2006-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	cotton (Gossypium hirsutum) senescence expressed sequenc tag (EST) senescence-associated genes suppression subtractive hybridization Chemistry/Food Science Geosciences Life Sciences Physics Engineering Science
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification and characterization of differentially expressed ESTs of Gossypium barbadense infected by Verticillium dahliae with suppression subtractive hybridization

Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton

Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton

Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton

Isolation and identification of genes expressed differentially in rice inflorescence meristem with suppression subtractive hybridization

Studies of new EST-SSRs derived from Gossypium barbadense

Rapid plant regeneration from cotton (Gossypium hirsutum L.)

Effects of pigment glands and gossypol on somatic cell culture of upland cotton (Gossypium hirsutum L.)

Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma

Identification of genes associated with cotyledon senescence in upland cotton

Similar Documents

Identification and characterization of differentially expressed ESTs of Gossypium barbadense infected by Verticillium dahliae with suppression subtractive hybridization

Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton

Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton

Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton

Isolation and identification of genes expressed differentially in rice inflorescence meristem with suppression subtractive hybridization

Studies of new EST-SSRs derived from Gossypium barbadense

Rapid plant regeneration from cotton (Gossypium hirsutum L.)

Effects of pigment glands and gossypol on somatic cell culture of upland cotton (Gossypium hirsutum L.)

Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma

Identification of genes associated with cotyledon senescence in upland cotton