NDLI: Potential existence of two independent centrosome-targeting domains in PP4

Content Provider	Springer Nature Link
Author	Huang, XiuQing Ning, LiFeng Long, ZhiTao Sun, LingLing Zhang, Hui Lian, MuLan Sang, JianLi
Copyright Year	2007
Abstract	Protein phosphatase 4 (PP4) is an important member in the PPP family of protein Ser/Thr phosphatases. It has been proven to regulate a variety of cellular processes such as centrosome maturation, microtubule nucleation, splicesome assembly, and JNK pathway activation. Compared to the crystallized and structurally well defined phosphatase PP1 and PP2B, little is known about the structure of PP4. Besides the conserved motifs characteristic of the PPP family, no information is available on the other domains of PP4. PP4 is reported to localize to the centrosome in many species such as Drosophila, Caenorhabditis elegans and mammalian cells, which suggests a conserved role of PP4 in the regulation of centrosome function. Unlike several other centrosomal proteins, no sequence has been identified for PP4 that can target it to specific centrosomal localization. In this study, we used a combination of PCR mutagenesis and transient expression of GFP-tagged proteins in mammalian cells, and identified two PP4 centrosome-targeting domains of 68–136 and 134–220 aa. These two domains may be associated for appropriate localization to the centrosome. The findings are useful for further elucidating the function of the domains and other structural characteristics of PP4.
Starting Page	2930
Ending Page	2937
Page Count	8
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	52
Issue Number	21
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2007-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	PP4 centrosome localization domain Science Physics Chemistry/Food Science Life Sciences Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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