NDLI: Calcium affecting protein expression in longan under simulated acid rain stress

Content Provider	Springer Nature Link
Author	Pan, Tengfei Li, Yongyu Ma, Cuilan Qiu, Dongliang
Copyright Year	2015
Abstract	Longan (Dimocarpus longana Lour. cv. Wulongling) of uniform one-aged seedlings grown in pots were selected to study specific proteins expressed in leaves under simulated acid rain (SiAR) stress and exogenous Ca$^{2+}$ regulation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that there was a protein band specifically expressed under SiAR of pH 2.5 stress for 15 days with its molecular weight of about 23 kD. A 17 kD protein band specifically expressed after SiAR stress 5 days. Compared with pH 2.5, the pH 3.5 of SiAR made a less influence to protein expression. Two-dimensional electrophoresis (2-DE) results showed that six new specific proteins including C$_{4}$ (20.2 kD pI 6.0), F (24 kD pI 6.35), B$_{3}$ (22.3 kD pI 6.35), B$_{4}$ (23.5 kD pI 6.5), C$_{5}$ (21.8 kD pI 5.6), and C$_{6}$ (20.2 kD pI 5.6) specifically expressed. C$_{4}$ always expressed during SiAR stress. F expressed under the stress of pH 2.5 for 15 days and expressed in all pH SiAR stress for 20 days. The expression of proteins including B$_{3}$, C$_{5}$, and C$_{6}$ was related to pH value and stress intensity of SiAR. The expression of B$_{4}$ resulted from synergistic effects of SiAR and Ca. The expression of G$_{1}$ (Mr 19.3 kD, pI 4.5), G$_{2}$ (Mr 17.8 kD, pI 4.65), G$_{3}$ (Mr 16.6 kD, pI 4.6), and G$_{4}$ (Mr 14.7 kD, pI 4.4) enhanced under the treatment of 5 mM ethylene glycol tetraacetic acid (EGTA) and 2 mM chlorpromazine (CPZ). These proteins showed antagonistic effects and might be relative to the Ca-calmodulin (Ca-CaM) system of longan in response to SiAR stress.
Starting Page	12215
Ending Page	12223
Page Count	9
File Format	PDF
ISSN	09441344
Journal	Environmental Science and Pollution Research
Volume Number	22
Issue Number	16
e-ISSN	16147499
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-04-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dimocarpus longana Lour Two-dimensional electrophoresis (2-DE) Simulated acid rain stress Protein expression Ca-calmodulin Exogenous Ca$^{2+}$ regulation Environment Environmental Chemistry Ecotoxicology Environmental Health Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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