NDLI: The roles of methanogens and acetogens in dechlorination of trichloroethene using different electron donors

Content Provider	Springer Nature Link
Author	Wen, Li Lian Zhang, Yin Pan, Ya Wei Wu, Wen Qi Meng, Shao Hua Zhou, Chen Tang, Youneng Zheng, Ping Zhao, He Ping
Copyright Year	2015
Abstract	We evaluated the effects of methanogens and acetogens on the function and structure of microbial communities doing reductive dechlorination of trichloroethene (TCE) by adding four distinct electron donors: lactate, a fermentable organic; acetate, a non-fermentable organic; methanol, a fermentable 1-C (carbon) organic; and hydrogen gas (H$_{2}$), the direct electron donor for reductive dechlorination by Dehalococcoides. The fermentable electron donors had faster dechlorination rates, more complete dechlorination, and higher bacterial abundances than the non-fermentable electron donors during short-term tests. Phylotypes of Dehalococcoides were relatively abundant (≥9 %) for the cultures fed with fermentable electron donors but accounted for only ~1–2 % of the reads for the cultures fed by the non-fermentable electron donors. Routing electrons to methanogenesis and a low ratio of Dehalococcoides/methanogenesis (Dhc/mcrA) were associated with slow and incomplete reductive dechlorination with methanol and H$_{2}$. When fermentable substrates were applied as electron donors, a Dhc/mcrA ratio ≥6.4 was essential to achieve fast and complete dechlorination of TCE to ethene. When methanogenesis was suppressed using 2-bromoethanesulfonate (BES), achieving complete dechlorination of TCE to ethane required a minimum abundance of the mcrA gene. Methanobacterium appeared to be important for maintaining a high dechlorination rate, probably by providing Dehalococcoides with cofactors other than vitamin B$_{12}$. Furthermore, the presence of homoacetogens also was important to maintain a high dechlorination rate, because they provided acetate as Dehalococcoides’s obligatory carbon source and possibly cofactors.
Starting Page	19039
Ending Page	19047
Page Count	9
File Format	PDF
ISSN	09441344
Journal	Environmental Science and Pollution Research
Volume Number	22
Issue Number	23
e-ISSN	16147499
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-08-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TCE Reductive dechlorination Dehalococcoides Methanogens Homoacetogens Environment Environmental Chemistry Ecotoxicology Environmental Health Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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