NDLI: Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay

Content Provider	Springer Nature Link
Author	Kao, Po Min Tung, Min Che Hsu, Bing Mu Chiu, Yi Chou She, Cheng Yu Shen, Shu Min Huang, Yu Li Huang, Wen Chien
Copyright Year	2013
Abstract	In this study, a SYBR green quantitative real-time PCR was developed to quantify and detect the Legionella spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and thermal spring area in Taiwan. Legionella was detected in 13.6 % (24/176), and the detection rate for river water, raw drinking water, and thermal spring water was 10, 21.4, and 16.6 %, respectively. Using real-time PCR, concentration of Legionella spp. in detected samples ranged between 9.75 × 10$^{4}$ and 3.47 × 10$^{5}$ cells/L in river water, 6.92 × 10$^{4}$ and 4.29 × 10$^{5}$ cells/L in raw drinking water, and 5.71 × 10$^{4}$ and 2.12 × 10$^{6}$ cells/L for thermal spring water samples. The identified species included Legionella pneumophila (20.8 %), Legionella jordanis (4.2 %), Legionella nautarum (4.2 %), Legionella sp. (4.2 %), and uncultured Legionella sp. (66.6 %). The presence of L. pneumophila in aquatic environments suggested a potential public health threat that must be further examined.
Starting Page	6128
Ending Page	6137
Page Count	10
File Format	PDF
ISSN	09441344
Journal	Environmental Science and Pollution Research
Volume Number	20
Issue Number	9
e-ISSN	16147499
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Legionella spp. Legionella pneumophila Real-time PCR Watershed Thermal spring Water treatment plant Environment Environmental Chemistry Ecotoxicology Environmental Health Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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