NDLI: Screening, breeding and metabolic modulating of a strain producing succinic acid with corn straw hydrolyte

Content Provider	Springer Nature Link
Author	Li, Xingjiang Zheng, Zhi Wei, Zhaojun Jiang, Shaotong Pan, Lijun Weng, Shibing
Copyright Year	2008
Abstract	Purpose In this paper, the high yield mutant strain was expected to be obtained for the industrial bioconversion of corn straw hydrolyte to succinic acid. Methods API biochemical reactions and 16S r RNA sequence analysis were carried out for identification, and then the strain’s metabolic pathway and its relevant enzymes were discussed for the metabolic flux analysis (MFA). The X-ray of synchronous radiation and site-directed mutagenesis were imported for decreasing those byproducts, and the metabolic technics was also modulated. Results Identification showed that the succinic-acid-producing strain S.JST belonged to Actinobacillus succinogenes species. Metabolic pathway analysis indicated that this strain had the character of utilizing glucose and xylose simultaneously, which was a great advantage considering the fact that most crop straw hydrolyte included glucose and xylose. Metabolic flux analysis showed that acetic acid and alcohol competed with the flux of succinic acid, and the analysis of [H] reducing power balance investigated that the [H] electronic donor produced in the cell was not enough for the metabolism of succinic acid. Then X-ray mutagenesis showed that the flux of byproduct acetic acid was decreased to 0.666 from 1.233 mmol/g DW h and that two mutated sites were found in pta (gene of phosphotransacetylase) from mutant strain M.JSTP, but less succinic acid was produced due to the worse lacking in the supply of [H] reducing power. The site-directed mutagenesis showed that the flux of byproduct alcohol was successfully decreased to 0.029 from 1.303 mmol/g DW h. Then, surrounding the flux ratio of HMP to EMP, the metabolic technics was modulated with the addition of citrate in order to improve the balance of [H] reducing power, thus the succinic acid flux of the mutant M.JSTA was increased to 3.163 from 2.480 mmol/g DW h comparing with the parent strain S.JST. Conclusions The mutant strain obtained in this paper deserves to be scaled-up.
Starting Page	667
Ending Page	677
Page Count	11
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	25
Issue Number	4
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-12-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Actinobacillus succinogenes Breeding Metabolic flux H reducing power Corn straw Microbiology Environmental Engineering/Biotechnology Applied Microbiology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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