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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mu, Li Wen, Jianping |
| Copyright Year | 2012 |
| Abstract | In the present work, Bacillus subtilis was engineered to produce l-malate. Initially, the study revealed that the slight fumarase activity under anaerobic conditions is extremely favourable for l-malate one-step fermentation accumulation. Subsequently, an efficient heterologous biosynthesis pathway formed by Escherichia coli phosphoenolpyruvate carboxylase and Saccharomyces cerevisiae malate dehydrogenase was introduced into B. subtilis, which led to 6.04 ± 0.19 mM l-malate production. Finally, the l-malate production was increased 1.5-fold to 9.18 ± 0.22 mM by the deletion of lactate dehydrogenase. Under two-stage fermentation conditions, the engineered B. subtilis produced up to 15.65 ± 0.13 mM l-malate, which was 86.3 % higher than that under anaerobic fermentation conditions. Though the l-malate production by the recombinant was low, this is the first attempt to produce l-malate in engineered B. subtilis and paves the way for further improving l-malate production in B. subtilis. |
| Starting Page | 33 |
| Ending Page | 41 |
| Page Count | 9 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 29 |
| Issue Number | 1 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-08-23 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | B. subtilis Heterogeneous biosynthetic pathway Lactate dehydrogenase l-malate Metabolic engineering Biochemistry Applied Microbiology Microbiology Biotechnology Environmental Engineering/Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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