NDLI: Expression of the guanosine-inosine kinase gene from Exiguobacterium acetylicum in Corynebacterium ammoniagenes and phosphorylation of inosine to produce inosine 5′-monophosphate

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Expression of the guanosine-inosine kinase gene from Exiguobacterium acetylicum in Corynebacterium ammoniagenes and phosphorylation of inosine to produce inosine 5′-monophosphate

Content Provider	Springer Nature Link
Author	Usuda, Yoshihiro Shimaoka, Megumi Kawasaki, Hisashi Utagawa, Takashi
Copyright Year	2010
Abstract	The guanosine-inosine kinase gene (gsk) isolated from Exiguobacterium acetylicum was expressed in an ATP-regenerating strain, Corynebacterium ammoniagenes. In order to induce its expression, three promoters (those for the Escherichia coli tac, Escherichia coli trp, and Corynebacterium glutamicum odhA gene) with the corresponding ribosome-binding sequences were examined. The E. coli trp promoter was most efficient with regard to inducing the expression of gsk in C. ammoniagenes. Further, the resulting strain, which has both inosine kinase activity and ATP-regenerating activity, was used to induce the phosphorylation of inosine to produce inosine 5′-monophosphate (5′-IMP), which is widely used as a flavor enhancer; approximately 80 g of 5′-IMP/l was produced with a molar conversion ratio of 80%.
Starting Page	709
Ending Page	712
Page Count	4
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	27
Issue Number	3
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-06-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Corynebacterium ammoniagenes Gene expression Guanosine-inosine kinase Exiguobacterium acetylicum Inosine Inosine 5′-monophosphate Microbiology Environmental Engineering/Biotechnology Applied Microbiology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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