NDLI: Production, purification and characterization of a thermostable laccase from a tropical white-rot fungus

Content Provider	Springer Nature Link
Author	Guo, Li Qiong Lin, Shuo Xin Zheng, Xiao Bing Huang, Zi Rou Lin, Jun Fang
Copyright Year	2010
Abstract	A thermostable laccase was isolated from a tropical white-rot fungus Polyporus sp. which produced as high as 69,738 units of laccase l$^{−1}$ in an optimized medium containing 20 g of malt extract l$^{−1}$, 2 g of yeast extract l$^{−1}$, 1.5 mM CuSO$_{4}$. The laccase was purified to electrophoretic purity with a final purification of 44.70-fold and a recovery yield of 21.04%. The purified laccase was shown to be a monomeric enzyme with a molecular mass of 60 kDa. The optimum temperature and pH value of the laccase were 75°C and pH 4.0, respectively, for 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS). The Michaelis–Menten constant (K $_{m}$) of the laccase was 18 μM for ABTS substrate. The laccase was stable at pH values between 5.5 and 7.5. About 80% of the initial enzyme activity was retained after incubation of the laccase at 70°C for 2 h, indicating that the laccase was intrinsically highly thermostable and with valuable potential applications. The laccase activity was promoted by 4.0 mM of Mg$^{2+}$, Mn$^{2+}$, Zn$^{2+}$ and Ca$^{2+}$, while inhibited by 4.0 mM of Co$^{2+}$, Al$^{3+}$, Cu$^{2+}$, and Fe$^{2+}$, showing different profiles of metal ion effects.
Starting Page	731
Ending Page	735
Page Count	5
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	27
Issue Number	3
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-07-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thermostable laccase Production and purification Enzymatic properties White-rot fungus Microbiology Environmental Engineering/Biotechnology Applied Microbiology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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